To efficiently exploit the approximately 44% remaining reserves in the main oil layers of Daqing Oilfield after polymer flooding, a polymeric surfactant composed of acrylamide (AM), dodecyl acrylamide, 2-acrylamido-2-methylpropanesulfonic acid and dodecyl polyoxyethylene (23) ether acrylate was prepared by using a method involving free-radical aqueous polymerization followed by weak alkaline hydrolysis. The application performance of polymeric surfactant A was compared and evaluated against commercial polymeric surfactant HB, and partially hydrolyzed polyacrylamide (PHPAM). Results demonstrated that the fundamental physicochemical properties of polymeric surfactant A meet the relevant technical standards. Under identical conditions, its viscosity-enhancing capability, salt resistance, and aging stability outperformed those of the comparative polymers. It can reduce oil-water interfacial tension and form an "O/W" (oil-in-water) emulsion with crude oil, with emulsification and water separation properties meeting the requirements of post polymer flooding reservoirs. In the natural Berea sandstone core oil displacement experiment at a concentration of 1000 mg/L and a polymer dosage of 0.5 PV, the enhanced oil recovery value after polymer flooding was 15.1%, which was 3 to 5 percentage points higher than those of the comparison polymers.