Gas chromatography technology was employed to screen solvents for extracting 38 types of oil-soluble tracers from crude oil. Methanol was selected as the tracer solvent due to its minimal extraction of crude oil components and the least signal interference. The aqueous solution formed by water in the oil and methanol severely affects tracer extraction, and residual crude oil components extend the analysis cycle to 10 hours. Therefore, further optimization of the tracer extraction method is needed. By adding 20% mass ratio of isooctane and excess K2CO3 to crude oil containing 1000 ppm tracers, and then using stirring, ultrasonic bath, and centrifugation to dehydrate the crude oil, followed by freezing the methanol extract at -32°C, tracer analysis in crude oil was conducted. This achieved precise separation and quantification of all tracers within 5 hours in the gas chromatogram. Experimental results demonstrate that using gas chromatography technology and solvent extraction can rapidly qualitatively and quantitatively analyze up to 38 oil-soluble tracers in crude oil in a single injection, thus monitoring crude oil flow and achieving reservoir characterization.