Affected by reservoir physical properties, there is a contradiction between the insufficient formation energy and difficult water injection in water flooding development of low permeability reservoirs. On the one hand, the water injection pressure is high and the underinjection is serious. On the other hand, the formation pressure drop of low permeability reservoirs is large, the productivity decreases rapidly, and the oil recovery rate and recovery degree are low. After water flooding, there are still many forms of residual oil such as membrane or oil droplets. In view of the above problems, this work synthesized metronidazole asymmetric Gemini surfactant, and combined with dehydro rosin surfactant, cetyl alcohol, ethanol to form a functional enhanced injection and oil displacement agent. The agent reduces the interfacial tension between oil and water, emulsifying crude oil and removing oil film. The cationic component forms molecular film on the rock surface with water as the transfer medium, stabilizes clay, improves the wettability of rock surface and reduces water injection resistance. Combined with the field test of pressure flooding, the daily oil increment of the well group is 13.4 t/d at the beginning and 12.6 t/d after 5 months. The injection capacity of the well was significantly improved. Before pressure flooding, the injection volume of the water well is 0 at the high pressure of 30 MPa, and 30 m³/d at 27.5 MPa after pressure flooding.