In view of the problem that the production of tight gas wells decreases due to water lock，and the development effect deteriorates，a water lock remover based on metabolites of hydrophobic modified Pseudomonas was prepared，and the surface/interface properties，wettability and spontaneous imbibition ability of the water lock remover were tested，and the effect of water lock removal was evaluated through the core displacement experiment. The results showed that the water lock remover could form a dense superhydrophobic biofilm in the gas reservoir，improve the relative permeability of the gas phase and reduce the relative permeability of the water phase. The 0.6% water lock remover could form a stable adsorption on the surface of the reservoir rock and restore the reservoir permeability of 56%—80%. After chemical treatment，the spontaneous liquid uptake and maximum liquid uptake rate of the treated core（about 60 g）decreased from 0.36 g and 0.05 g/min to 0.16 g and 0.01 g/min，respectively. The simulation experiment of core displacement showed that the water lock remover could effectively reduce the relative permeability of water phase and increase the relative permeability of gas phase，which was mainly reflected in the increase of the water-phase relative permeability shape factor and the decrease of its endpoint value，as well as the decrease of the gas-phase relative permeability shape factor and the increase of its endpoint value. The water lock remover had good adaptability to the water lock damage of the four gas field reservoirs，which could effectively remove the water lock and restore the reservoir permeability.