Aiming at the problem of low liquid and low yield in Zhuang 139 block wells in deep heavy oil reservoir，which did not get good results of the second cycle of DC as a result of high pressure of steam injection，the technology for microbial huff and puff in deep heavy oil reservoir was studied. According to the reservoir characteristics and crude oil properties of test well，the main components of microbial complex huff and puff system were optimized，which was applied to Zhuangxi oilfield and Taiping oilfield by designed injection slug. The results showed that by using microbial compound system that consisted of activator II（0.4% molasses+0.2% corn pulp dry powder+0.2% dextrin+0.2% NaNO₃ +0.02%（NH₄ ） 2HPO₄ ），inoculating microbes containing bacillus polymyxa. and 0.1% surface active agent of Sophora japonica lipid biologic surfactant，the effect of emulsified crude oil was good and gas production was great under the pH value of 6. The viscosity of crude oil reduced by 50.1%，the oil increase period was 166d，the cumulative oil increased 915 t，the input-output ratio was 1∶9.07，and the economic benefit was obviously improved after microbial compound system was injected in field with 6 m3/h injection speed and 200 m3 main slug