As an unconventional oil resource，oil sands and its properties ，separation methods had attracted more and more attention both in china and abroad. In this paper ，the effect of wettability of oil sands on the bitumen separation processes was discussed. The methods for the determination of wettability of oil sands and the influence factors during the determination processes were introduced. The effect of weathering on the wettability of oil sands was analyzed. The effect of wettability on the processability of oil sands was also discussed briefly.