The silicon molybdbenum yellow method for spectrophotometry determination was applied to quantitative analyze silicon ion in ASP-flooding produced water. The effects of NaOH, polymer (HPAM) and sodium dodecylbenzene sulfonate (LAS) on quantitative analysis of silicon ions were discussed. The results showed that NaOH (0—7200 mg/L) and LAS (0—2400 mg/L) had almost no effect on the absorbance. Hydrochloric acid could be used to eliminate the influence of NaOH with high concentration (>7200 mg/L). When the pH value was 1.2 and the dosage of HPAM (M=35 million) was between 0 and 80 mg/L, solution absorbance increased with increasing concentration of HPAM. The interference of polymer on the measuring of silicon ion could not be eliminated by adding 1 mL acetone, but it could be eliminated by diluting with distilled water. The interference of polymer with low molecular weight (M=3 millon) and low concentration (0—40 mg/L) was weak. The method suggested was simple, rapid and of satisfactory precision and accuracy. The standard curve of linear regression equation was: A=0.0145c, and the correlation coefficient R2 was 0.9996. The linearity of absorbance concentration relationship maintained in the range of silicon ion concentration 0—20 mg/L. The accuracy of the method was less than 2%. The measured value of the relative standard deviation was only 1.39%, which indicated the repeatability of this method was good.