In order to obtain polymer microsphere for profile control and water shutoff with good suspension，high temperature resistance and high salinity tolerance，nanometer or micrometer polymer microsphere DCA which had a three-layer structure was synthesized by the monomer of acrylamide，initiator of azobisisobutyronitrile，crosslinking agent of divinylbenzene and emulsifier of ethoxylated lauryl alcohol sulfate. The basic performance of DCA microsphere was evaluated，including temperature resistance， suspension，expansion and plugging effect. The results showed that the particle size of DCA microsphere ranged from 0.1—30 μm and the shell had a nanoporous structure. The temperature resistance of DCA microsphere was up to 300℃，and DCA microsphere could be placed 90 days at 115℃. The sedimentation rate of DCA microsphere in simulated formation water with 28 hrs standin time was 0.005 mL/min，showing good suspension. The expansion ratio of DCA microsphere increased with increasing temperature. The maximum expansion ratio of DCA microsphere soaked 24 hrs at 115℃ in simulated formation water was 13.83%. After the experiment of profile control and water plugging with three layer heterogeneous cores，the amplitude of enhanced oil recovery was up to 7% and water-cut rate reduced by 5%. Domestic oilfield test showed that the liquid-producing capacity and water-cut rate reduced and oil increment each day was obvious after microsphere slug profile control. DCA microsphere was suitable for the deep profile control and water shutoff of high temperature and high salinity reservoir.