According to the nutritional requirements of the main oil recovery functional bacteria in the sandstone reservoir of LQ well block, the optimal carbon source, nitrogen source and phosphorus source were selected through single factor experiment, and the concentration of each component was preliminarily determined, on this basis, the significant influence factors of each component were analyzed by Plackett-Burman experiment and factor removal experiment, and the concentration of each component was determined, finally, response surface test was used to determine the concentration of each component, the final concentration was obtained by further optimizing each component according to the factors. The results showed that the selected nutrient system had good emulsifying effect, the main oil recovery function gene hydrocarbon oxidation gene reached 10_⁷copies/ml, and the physical model oil displacement experiment improves oil recovered by 11.65%. This study provides a good technical support for the field test of microbial flooding in well block LQ.