The adsorption and retention of nanofluids on the surface of porous media are critical factors influencing the oil recovery enhancement in unconventional oil and gas recovery by nanotechnology. As a kind of amphiphilic sheet nanomaterials, it is necessary to study the dynamic adsorption and desorption of nanosheet in the cores. The standard curve of nanosheet fluid was plotted and the static adsorption experiment was carried out to clarify the concentration at which it reached adsorption saturation. On this basis, the dynamic adsorption experiments of nanofluids with different influencing factors were carried out. Afterwards, the variation laws of dynamic adsorption capacity were investigated by single-factor analysis and multi-linear regression method, and then the regression equation of dynamic adsorption capacity was established. The results of laboratory experiments indicated that the saturation adsorption concentration of the nanofluid was 75 mg/L. The dynamic adsorption capacity has a negative logarithmic correlation with the permeability of low permeability cores, a negative linear correlation with the salinity degree, and a positive linear correlation with the injection amount of the nanofluid, as well as the injection rate of the subsequent water flooding. The multiple linear regression equation could explain 98.9% of the variation in the dynamic adsorption capacity. Therefore, maintaining a moderate salinity range, adjusting the slug level of nanofluid, and reducing the injection rate of water flooding injection, can mitigate the dynamic adsorption capacity, which is beneficial for improving oil recovery in low-permeability reservoirs.