In order to explore the reservoir damage characteristics of shale oil CO₂ flooding，based on the natural core and shale oil samples of a shale oil reservoir in the west，the shale oil reservoir damage caused by gas channeling，asphaltene deposition and Jamin effect was studied through laboratory experiments. Through permeability change analysis，nuclear magnetic resonance response and contact angle test，the intervention effects of various micro-regulation methods on typical damage mechanisms were explored. The results showed that gas channeling made gas breakthrough in high permeability layer，which led to the decrease of overall recovery rate during commingled production. The influence of asphaltene deposition on low permeability cores was more obvious，mainly concentrated on the injection side. The Jamin effect led to a decrease in permeability when the downstream back pressure was lower than the miscible pressure. The high permeability core was less affected，while the low permeability core was greatly affected and was not easy to be relieved. The measures and suggestions for reducing reservoir damage such as gas-water alternation，anti-deposition of gas injection wells，and change of production pressure difference were given，which provided theoretical support and technical guidance for the efficient development of shale oil by CO₂ flooding.