The adsorption and retention of nanofluids on the surface of porous media are critical factors influencing the oil recovery enhancement in unconventional oil and gas recovery by nanotechnology. As a kind of amphiphilic sheet nanomaterials，it is necessary to study the dynamic adsorption laws of nanosheet in the cores. Firstly，the standard curve of nanosheet fluid was plotted and then the static adsorption experiment was carried out to clarify the concentration at which it reached adsorption saturation. On this basis，the dynamic adsorption experiments of nanofluids with different influencing factors were carried out. Afterwards，the variation laws of dynamic adsorption capacity were investigated by single-factor analysis and multi-linear regression method，and then the regression equation of dynamic adsorption capacity was established. The results showed that the saturation adsorption concentration of the nanofluid was 75 mg/L，while the maximum static adsorption capacity was 1.83 mg/g. The dynamic adsorption capacity had a negative logarithmic correlation with the permeability of low permeability cores（K），a negative linear correlation with the fluid mineralization ratio（MS），and a positive linear correlation with the injection slug of nanofluid（V₂），as well as the injection rate（v）of the subsequent water flooding. The multiple linear regression equation between the dynamic equilibrium adsorption capacity（ η₂ ）and K，MS，v and V₂ of the nanosheet fluid in the low permeability core was obtained as follows： η₂ = 10^-3(-0.4 - 0.005 K - 0.094 MS + 1.301 v + 3.347 V₂) ，with R²=0.989. Under the condition of 20×10^-3 μm²（K），2（MS），0.3 mL/min（v），and 0.5 PV（V₂），the dynamic adsorption capacity of the nanosheet was 1.328×10^-3 mg/g by regression equation，closing to the experimental result（1.321×10^-3 mg/g）. Maintaining a moderate salinity range（MS＜4），adjusting the slug level of nanofluid （0.5 PV），and reducing the injection rate of water flooding（＜0.45 mL/min），could mitigate the dynamic adsorption capacity， which was beneficial to further improve oil recovery in low-permeability reservoirs.