In order to effectively improve the problem of production decrease year by year due to water injection and gas injection development in fractured-vuggy reservoirs，Tahe oilfield，three equally scaled 3D visual physical models of fractured-vuggy reservoirs were created through 3D printing，including weathered crust，fault-controlled karst，and underground river reservoir models. Subsequently，the microstructure of the black nano-sheet was characterized through TEM and AFM. The oil-water interface adsorption，wetting reversal property and oil displacement effect of the black nano-sheet in three physical models were studied. Based on the actual situation in the field，two types of typical structural unit well groups，such as inter-well and single-well were selected to carry out field pilot tests. The results showed that the average size of black nano-sheet was about 80×60×1.2（nm），and the specific surface area was large. It had good amphipathic property and then could form a stable adsorption layer at the oil/water interface. It had good wetting reversal property and then could change the surface of quartz plate from oil to water. In the 3D models of weathered crust，underground river and fault-controlled karst，black nano-sheet flooding could increase crude oil recovery by 11 percentage point，8 percentage point and 7 percentage point respectively on the basis of water flooding. The weathered crust reservoir had the most obvious benefit effect. Besides，field test results of 15 rounds showed that black nano-sheet flooding was not suitable for single-well replacement production methods in three karst reservoir spaces. The well group composed of weathered crust and underground river karst reservoir spaces had good inter-well connectivity and high acceptance，which was the optimum application area in-situ. In the area，the efficiency was as high as 85.7%，and the oil increase was significant with a cumulative oil increase of about 5000 t. The research results provided on-site guidance and theoretical basis for the subsequent development of black nano-sheet flooding technology in fractured-vuggy reservoirs in Tahe oilfield.