In order to obtain a strain that could produce bioemulsifier and degrade crude oil，the bacteria that could emulsify crude oil was selected using crude oil as the sole carbon source，and the production of bioemulsifiers by Pseudomonas YZ32 was optimized by adjusting the composition of the culture medium. The optimal carbon and nitrogen sources were selected based on the emulsification index and emulsifier yield. bioemulsifiers was extracted with ethanol and purified with dialysis bags，the sugar and protein content of the emulsifiers was measured，the emulsifying active substances of the emulsifiers was tested. By Mixxing bioemulsifiers with 7 kinds of different substrates and measuring the emulsification index，the bioemulsification effect of emulsifiers on different substrates was investigated. Under different conditions，the physicochemical stability of bioemulsifiers was investigated. The crude oil degradation experiments were carried out，the components of crude oil were analyzed using GC-MS to investigate the removal rate and degradation effect of strains on crude oil. The results showed that a strain of Pseudomonas YZ32 capable of emulsifying crude oil was isolated. The maximum bioemulsifier yield could be obtained using 20 g/L of sucrose and 7 g/L of sodium glutamate as carbon and nitrogen sources for YZ32. The protein content of its metabolites was 64.29% and the sugar content was 13.89%，and the emulsifying active substances were protein-like substances. The bioemulsifier had a good emulsifying effect on liquid paraffin and toluene. The bioemulsifier produced by YZ32 could maintain good stability under the temperatures of 15—80 ℃，pH value of 4—11 and salinity of 50—350 g/L. The crude oil degradation experiment showed that YZ32 achieved 60.3% removal of crude oil within 24 d with better degradation effect for C₁₁—C₂₀ components in saturated hydrocarbons and phenanthrene in aromatic hydrocarbons of crude oil. Pseudomonas YZ32 could produce stable protein based bioemulsifiers. YZ32 had a good degradation effect on crude oil and had great potential for application in the remediation of oily sludge soil.