The asphaltene deposition of crude oil caused by CO₂ injection will damage the tight reservoir and seriously affect the ultimate recovery. In order to clarify the characteristics of asphaltene deposition and the influence on reservoir permeability under different CO₂ injection methods，the experiments of CO₂ flooding and CO₂ huff-n-puff were carried out. The characteristics of asphaltene deposition，the performance of oil recovery，and the influences on the permeability of tight cores under different injection methods were analyzed by nuclear magnetic resonance（NMR）and scanning electron microscope（SEM）. The results showed that the asphaltene deposition occurred in the tight cores under both of CO₂ flooding and huff-n-puff，which was mainly deposited on the pore surface by membrane adsorption. Influenced by the interaction time between CO₂ and crude oil，the amount of asphaltene deposition under CO₂ huff-n-puff was greater than that under CO₂ flooding. Moreover，the pore size range of asphaltene deposition under CO₂ huff-n-puff was higher than that of CO₂ flooding. The damage of asphaltene deposition to core permeability was related to the direction of crude oil production. Asphaltene deposition had greater damage to the forward（CO₂ injection direction）core permeability under CO₂ flooding，while that had greater damage to the reverse core permeability under CO₂ huff-n-puff. When the confining pressure was 10 MPa and 5 MPa，the damage of asphaltene deposition under CO₂ flooding and CO₂ huff-n-puff to the forward core permeability（average decrease of forward permeability）was 7.05% and 1.67%，while the damage degree of the reverse core permeability（average decrease of reverse permeability）was 0.41% and 2.66%，respectively. Due to the influence of injection-production mode and flow mechanisms，the recovery of CO₂ huff-n-puff was lower than that of CO₂ flooding. The research results were significant in understanding the damage caused by asphaltene deposition in different directions and selecting injection methods during CO₂ injection in tight oil reservoirs.