In order to clarify the law of oxygen consumption during air or oxygen-reduced air flooding，the static oxidation experiment was used to emulate the oxygen consumption process in single-factor and multi-factors oxidation reactions of crude oil under actual reservoir conditions，then quantitative characterization of the static oxygen consumption was obtained. What’s more， slimline air flooding experiment was performed to analyze the law of dynamic oxygen consumption on-way. The results showed that the oxygen consumption due to the low-temperature oxidation（LTO）reaction of crude oil would decrease the total pressure of the system. The oxygenation and bond-breaking reactions of hydrocarbon occurred to produce CO₂ and CH₄. As per the calculation from the process of oxygen consumption in actual reservoir stratum，oxygen of LTO consumption occupied 87.84% of the total consumption，dissolve in water occupied 6.3%，while oxygen consumption of the reducing substances in formation water and core was 5.86%. Moreover，consumption of on-way oxygen occurred，meanwhile the propelling in front of oxidation was observed. Relatively high temperature and pressure as well as the saturation of oil would intensify the degree of LTO，thus increasing oxygen consumption. The conclusion provided a reference and guidance to the safe and efficient application of air/oxygen-reduced air flooding in the reservoir.