With the development and application of volumetric hydraulic fracturing in horizontal wells，more and more fracturing fluid is being used and a lot of flowback fluid is produced. The composition of flowback fluid is complex，and It's hard to use efficiently. Direct discharge will cause environmental pollution. In order to relieve the shortage of water and reduce the fracturing cost in Xinjiang oilfield，the reutilization technology of flowback fluid from Jimusaer Shale oil fracturing was studied. After chemical treatment，such as pH adjustment，boron ion shielding and sterilization，the fracturing flowback fluid of Jimsar shale oil block was used to prepare guar gum fracturing fluid. Through investigating the swelling performance，the temperature and shear-resistance and the gel breaking performance，the best formula of guar gum fracturing fluid was determined. Then the field test was carried out at well J1. According to the characteristics of high alkali content，high boron content and high bacteria content of Jimsaer shale oil flowback fluid，the injection of 0.06% pH regulator A，0.08% shielding agent C and 0.10% efficient fungicide BLX-1 was carried out，the pH of the flowback fluid was adjusted to 7.0；then 0.3% cross-linking agent XJ-3 and 0.045% pH regulator B were added. the cross-linking time of the fracturing fluid prepared with the treated flowback fluid could be controlled within 90—110 s，and the viscosity of the fracturing fluid after gelation was still greater than 200 mPa·s after shearing for 120 min， indicating that the fracturing fluid had good performance of temperature tolerance，shearing resistance and proppant carrying capacity. Furthermore，it also possessed perfect gel breaking performance and could meet the industry standard. By using continuous mixing and reuse technology of shale oil fracturing fluid，4.5 ×10⁴ m³ fracturing flowback fluid was reused，moreover，the prepared fracturing fluid had successfully applied to shale oil well J1 of Xinjiang oilfield，which layed the groundwork for efficient and environmentally friendly development of shale oil in future.