In order to regulate the test method of interfacial tention for the non-Newtonian fluid in industry standard of the SY/T 5370—2018“Test method for surface tension and interfacial tention”，the influence factors of oil and water interfacial tension in compound flooding such as temperature，rotation speed，concentration of the non-Newtonian fluid and polymer molecular mass，testing time and surfactant concentration were regulated. It was suggested that the interfacial tension between the complex flooding and the crude oil should be measured under the optimal conditions that measurement temperature was the target reservoir temperature，the rotation speed was 5000~ 6000 r/min，at which the instrument could produce clearly image，and the polymer concentration was 0.1%，the molecular mass of the polymer should be chosen according to the property of the target reservoir，however，it was recommended to apply the polymer with the molecular mass of 10 million to 15 million during arbitration or sampling. The interfacial tension value was determined according to equilibrium interfacial tension at different time，and when retraction or fracture happened during the measurement process，the interfacial tension before retraction or fracture should be measured three times and calculated mean value. When the surfactant was evaluated，the SP flooding system of 0.2% surfactant+ 0.1% polymer was recommended，meanwhile，the ASP flooding system of 1.2% alkali+0.1% surfactant+0.15% polymer and 0.6% alkali+0.3% surfactant +0.15% polymer were recommended.