Dual polarization interferometry was used to investigate the interaction between typical Daqing crude oil and three acrylamide-based polymers，and the effects of polymer concentrations and structures on the mass，thickness and density of oil films were examined. A five-stage model，including physical absorption，high coverage，deposition of polymer micelles，solubilization of crude oil and encapsulation of oil drops was proposed for the interaction between amphiphilic polymer and oil . It was found that NaCl solution has a negligible effect on mass of oil film. The mass and thickness of oil film increased and the density of oil film decreased after eluted with the amphiphilic polymeric surfactant PAM1 solution，because the formed polymer micelles could dissolve oil and carry it away from oil film. The higher the concentration of PAM1 was，the stronger the interaction was. PAM2， bearing a side unit of 2-Acryloylamino-2-methyl-1-propanesulfonic acid，could only adsorb onto the surface of oil film；while PAM3，a common HPAM，showed no interaction with crude oil owing to its strong hydrosolubility.