In order to improve the determination accuracy of partially hydrolyzed polyacrylamide（HPAM）in produced liquid by liquid chromatography，the effect of HPAM relative molecular weight，molecular weight distribution，hydrolysis degree，solution salinity and other factors on the determination results was studied，and the problems existing in the determination of HPAM concentration in produced fluid on site was analyzed. The results showed that the polymer relative molecular weight，molecular weight distribution and solution salinity had no significant impact on the concentration determination，while the hydrolysis degree was the restriction factor of polymer concentration determination in produced fluid by liquid chromatography. The slope of work curve increased with increasing hydrolysis degree of HPAM. When the hydrolysis degree of standard sample was lower than that of the sample to be tested，the measured result was lower than the true one，on the contrary，that was higher. After tested sample on site was pretreated by microporous membrane with suitable pore size and dilution method with distilled water，the interference effect of the oil in produced fluid sample，organic impurities and higher chromaticity could be eliminated. Because the polymer dry powder sample for injection as standard sample on site measurement，the measured result of polymer concentration was lower than the actual value.