The conventional metal crosslinked fracturing fluid system have some weakness, such as great toxicity and the produced second pollution to the formation duiring crosslinking process at alkaline condition;while the non-metal ion crosslinked fractureing fluid system also has some problems, such as slow gelling rate and poor shear resistance performance. In the paper, a low toxicity fracturing fluid with a novel cross-link agent system was prepared, the best formula of the fracturing fluid were obtained by analyzing the main factors affecting gel performance, the high temperature and shearing resistance and demulsification performance were investigated. The experimental results showed that, when the thickener content was 0.15%, the amino sulfonic acid content was 0.15%, the ethanol content was 1%, the of coagulator content was 0.020%—0.025%, the crosslinking agent content was 0.25% and the potassium chloride content was 0—2%, the fracturing fluid with the excellent performance could be prepared. the gelling time of the optimal fracturing fluid was about 150 s, and the initial viscosity after gelling was of 1529.0 mPa·s. When the fracturing fluid was continuously sheared at the temperature of 100℃ and the shearing rate of 170 s^-1 for 60 minutes, 90 minutes and 120 minutes, respectively, the viscosity was of 259.9 mPa ?s, 197.4 mPa ? s and 166.8 mPa ?s, respectively，indicating that the fracturing fluid had strong high temperature and shearing resistance. The gel can be broken totally with 0.03% of potassium persulfate at the temperature of 90℃within 3 hours. The gel-broken fluid was clear, and the viscosity was only 1.07 mPa·s. The fracturing fluid system did not contain any toxic metal ions, reducing the damage to formation.