In this paper, the adhesive bacteria isolated from the production water in Daqing oilfield was used to investigate the movement of bacteria and the hydrocarbon adhesion by the method of microscopic observation and micro- bial adhesion to hydrocarbons (MATH) method. The results showed that the oil adhesive effection of adhesive bacteria could be formed within 10 min, at the same time, the chemotaxis phenomena could be observed notably. on logarithm growth phase of bacteria, the average surface hydrophobicity value of the strains PR-1、1507、12-J to xylene, hexadecane, dodecane and cyclohexane, were 83%、56% and 21%,respectively. A certain concentration of organism A (biological surfactant) and B(chemical surfactant) played an significant role on the microbial chemotaxis and adhesive effection. After adding 0.01 mol/L of A or B, The surface hydrophobicity value of strain 12-J was increased from 18% to 70% and 85%, respectively, which obviously stimulated the chemotaxis movement of the adhesive bacteria. The further microscopic observation showed that the addition of a certain amount of organism A and B could improve significantly the chemotaxis movement of the adhesive baterica to the crude oil.