With the increasing attention to environmental protection and reservoir protection in domestic oil fields, the green non-toxic self-degrading temporary plugging agent has become a research focus in recent years. A new self-degrading water-soluble temporary plugging agent was developed by combining two kinds of self-degrading materials and the formula was 60%PA+40%YG-1. The dissolution rates of the temporary plugging agent in formation water, liquid preparation water and fracturing fluid within 8 hours were all more than 95% and the compatibility was good. The plugging rates of the temporary plugging agent to fractured core were more than 80% in 50℃-80℃. The broken rate of the temporary plugging agent was less than 10% at 40MPa. The temporary plugging agent has good dispersion and suspension performance in guar gum base solution and it has little influence on the rheologic property of fracturing fluid. It can be concluded through GC-MS that the temporary plugging agent hydrolysate contains only glucose and glycollic acid and has no pollution to the environment. The new temporary plugging agent has been successfully applied in 32 wells in a middle and high water-cut block of Changqing low permeability reservoir and the results show that the new temporary plugging agent can effectively increase the fracture net pressure and open new fractures. Compared with the conventional temporary plugging agent, the new temporary plugging agent has better stimulation effect and the advantage of environmental protection, having a broad application prospect.