Aiming at the problem that common surfactants didn’t resist high concentration salt and it was not easy to be used as flooding chemicals and fracture liquids in high salinity reservoirs，a new type of betaine surfactant containing hydroxyl and sulfo， named N-（6-tetradecyloxy-5-hydroxyl）-propoxyethyl-N-dimethyl-N-（2-hydroxyl）propanesulfonic acid sodium ammonium chloride， was successfully synthesized by two step etherification and one quaternization with tetradecyl alcohol，epoxy chloropropane，N，N-dimethylethanolamine and 3-chloro-2-hydroxy propanesulfonic acid sodium as main raw materials. The structure of the product was characterized by infrared spectrometer，and their surface activities of surfactant solution were studied. The results showed that the synthesized surfactant had good surface activity，whose critical micelle concentration（c_CMC）was 6.4 × 10^-4 mol/L，surface tension（γ_CMC）was 32.2 mN/m，surface tension reduction efficiency（pC₂₀）was 3.38，the saturated extent of adsorption（Γ_max）was 2.80×10^-6 mol/m² and the minimum adsorption area（A_min）was 0.17 nm² per molecule at 25℃. The high salinity had less impact on the hydrophilic group of surfactant. When the mass concentration of NaCl，CaCl₂ and MgCl₂ arrived 2.8×10⁵，2.0×10⁴ and 2.0×10⁴ mg/L，respectively，the synthesized Gemini surfactant didn’t generate precipitation，indicating good salt resistance.