文章摘要
菌株BIT-BP004产胞外多糖影响因素及发酵液理化特性的研究
Study on the Optimum Fermentation Condition and Physico-Chemical Property of Extracellular Polysaccharide Producing Strain BIT-BP004
  
DOI:
中文关键词: 胞外多糖  条件优化  正交实验  发酵液黏度  理化特性
英文关键词: extracelluar polysaccharide  optimal condition  orthogonal experiment  viscosity of the fermentation broth  physico-chemical property
基金项目:
作者单位
汪小平,付娜,王平,赵静,梅晓丹 1. 中国石油辽河油田金马油田开发公司工艺研究所,辽宁盘锦,124010
2. 大连百奥泰科技有限公司研发中心
,辽宁大连,116025 
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中文摘要:
      从辽河油田油水样品中分离得到一株高产胞外多糖的菌株BIT-BP004。通过对其培养条件的考察和优化。得到最适宜的培养条件为:蔗糖7.5g/L。NaNO30.3g/L,l(2HPO41.5g/L,KI-12PO43g/L,MgSO40.1g/L,CaCl20.01g/L,初始pH为6,接茵量1%。培养24h后的胞外多糖产量为4.11g/L。同时,通过正交实验考察了温度、矿化度和pH值对BIT-BP004发酵液理化性能的影响。结果表明,不同实验条件对发酵液黏度影响较大。影响能力大小依次为温度〉矿化度〉pH,其中温度是影响发酵液黏度的决定性因素。图5表5参10
英文摘要:
      An extracellular polysaccharide(EPS) producing bacteria,named BIT-BP004,was isolated from oil/water sample of Liaohe oilfield.The optimal medium compositions in shake scale for strain BIT-BP004 were determined as follows: sucrose 7.5 g/L,NaNO3 0.5 g/L,K2HPO4 1.5 g/L,KH2PO4 3 g/L,MgSO4 0.1 g/L,CaCl2 0.01 g/L,initial pH 6 and inoculant’s volume 1%.The EPS yield was 4.11 g/L in batch culture after 24 h.By orthogonal design,the factors of temperature,mineral salt concentration and pH value were selected to assay the physico-chemical property of fermentation.The results showed that experiment factors had a larger effect on viscosity of fermentation,and the affection capacity was arranged as follows,temperature > mineral salt concentration > pH,indicating that temperature was a decisive factor on the viscosity of the fermentation broth.
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